Lab Report

Cory Smith Date performed-14-Nov-2011 Lab session: Tuesday Evenings 6 pm Lab #18 Extraction of DNA from W point fire Germ Purpose The purpose of this experiment is to call up down DNA from w light up germ. The experiment will affair heat and enzymes to denature proteins and laundry detergent to open prison cell and membranes. Method ignite to denature proteins: 1. implement a weighing dish and an electronic balance and obtain 0.5g of raw down wheat germ. ordain that strain into 50ml beaker. 2. Put about 50 ml distilled body of water into a 250 ml beaker and heat until 55 degrees Celsius no to a greater extent(prenominal) than 60 degrees Celsius. Stir water periodically and monitor temperature. 3. Use 50 ml graduated piston chamber to put 10 ml of heated water into beaker containing sample from step 1. birl coalesceture for three minutes. 4. Add 10-12 drops of liquidise dishwashing detergent to the mix in step 3 then twistin g for another 5 minutes. 5. Let beaker sit for 1-3 minutes to seize the solid material to settle to bottom, exit difficult liquid above it. 6. Using a scavenge plastic eye eye dropper remove sufficiency of cloudy liquid from the beaker to fill 10 cm adjudicate thermionic valve 1/3 sufficient. dip the tribulation resistance and use a plastic dropper to remove some(prenominal) foam or bubbles that may induct formed on top of the cloudy liquid. 7.

Put 4-ml of alter inebriant into a 10ml graduated cylinder. 8. Tilt the test tube and slowly teem the alcohol down the sides so the alcohol forms a bed on top of cloudy liquid. Do not mix the layers toge! ther. 9. The DNA released from the wheat germ cells is in the lower, water containing cloudy layer. The DNA is not soluble in alcohol. Precipitate more DNA from the water layer by carefully inserting wooden splint through the alcohol layer to bottom of test tube. Pull the wooden splint up to the top and bring up the DNA precipitate. Effects of detergent on cell membranes: 1. review the steps of Part A, except do not do step 4. Record results in display board and be sure to note differences....If you want to get a full essay, order it on our website: BestEssayCheap.com

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